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rhodamine conjugated wga  (Vector Laboratories)


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    Structured Review

    Vector Laboratories rhodamine conjugated wga
    Rhodamine Conjugated Wga, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rhodamine+conjugated+wga/Rhodamine+labeled+Wheat+Germ+Agglutinin+(WGA)/10__3390_slash_ijms27010256-219-5-7
    Average 96 stars, based on 266 article reviews
    rhodamine conjugated wga - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Whole Genome Amplification:

    Article Title: Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division.
    Article Snippet: LipidSpotTM 488/610 Lipid Droplet Stains (#70065/#70069) and CF®488A, CF®640R or CF®555-conjugated wheat germ agglutinin (WGA; #29022, #29026 and #29076, respectively) as well as MitoViewTM Fix 640 (#70082), were obtained from Biotium Inc. (Fremont, CA). .. In some experiments Rhodamine-conjugated WGA (#RL-1022) from Vector Laboratories (Burlingame, CA) was used. .. Alexa Fluor®546 phalloidin (#A22283) was from Thermo Fisher Scientific.

    Article Title: Exosomal NAMPT from Engineered Mesenchymal Stem Cells Mitigates Aortic Stenosis via Metabolic and Anti-Inflammatory Pathways
    Article Snippet: .. The primary antibodies used are rhodamine-conjugated WGA (Vector Laboratories, Newark, CA, USA, RL1022, 1:250), tdTomato (ORIGENE, Rockville, MD, USA, AB8181200, 1:200), Griffonia Simplicifolia Lectin I Isolectin B4 Fluorescein (Vector Laboratories, Newark, CA, USA, FL-1201, 1:50) and Alpha-SMA (Thermo Fisher Scientific, Waltham, MA, USA, PA5-85919, 1:200). .. After washing, the sections were incubated with the secondary antibodies at room temperature for 1 h. The secondary antibodies used are Rabbit anti-Goat IgG, Alexa FluorTM 488 (Thermo Fisher Scientific, Waltham, MA, USA, A11078, 1:250) and Goat anti-Rabbit IgG, Alexa FluorTM 594 (Thermo Fisher Scientific, Waltham, MA, USA, A11037, 1:250).

    Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC-CXCL1-CXCR2 axis.
    Article Snippet: For PDGFRα staining, a secondary antibody (goat anti-rabbit IgG [H + L] conjugated to Alexa Fluor 594 (1:200; A11012, Thermo Fisher Scientific)) was used, followed by incubation for 1 h at room temperature (20–25 °C). .. Rhodamine-conjugated WGA (1:200; RL-1022-5; Vector Laboratories) was used to visualize plasma membranes. .. Sections were mounted with DAPI (1:1,000; D1306, Thermo Fisher Scientific) and incubated for 10 min at room temperature.

    Article Title: Small molecule stimulators of steroid receptor coactivator-3 and methods of their use as cardioprotective and/or vascular regenerative agents
    Article Snippet: After that, antigen retrieval was performed (Antigen unmasking solution, Tris-based, Vector Labs cat #H-3301; Vector Labs, Burlingame, CA). .. Sections were permeabilized with 0.1% tween20-PBS, blocked with 10% donkey serum in 1% tween20-PBS, and then incubated with primary antibody in blocking solution (1:200 Rabbit anti-Lysozyme, abcam cat #AB108508; Abcam, Cambridge, United Kingdom), followed by secondary (1:200 Donkey anti-Rabbit, Alexa 647, Thermo Fisher Scientific cat #A-31573; Thermo Fisher Scientific, Waltham, MA), and then Rhodamine-conjugated WGA (1:250 Vector Labs Cat #RL-1022) and DAPI (1:500 Thermo Fisher Scientific Cat #62248). .. Images were taken on a Zeiss LSM780 confocal microscope.

    Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC–CXCL1–CXCR2 axis
    Article Snippet: For PDGFRα staining, a secondary antibody (goat anti-rabbit IgG [H + L] conjugated to Alexa Fluor 594 (1:200; A11012, Thermo Fisher Scientific)) was used, followed by incubation for 1 h at room temperature (20–25 °C). .. Rhodamine-conjugated WGA (1:200; RL-1022-5; Vector Laboratories) was used to visualize plasma membranes. .. Sections were mounted with DAPI (1:1,000; D1306, Thermo Fisher Scientific) and incubated for 10 min at room temperature.

    Article Title: Exosomal NAMPT from Engineered Mesenchymal Stem Cells Mitigates Aortic Stenosis via Metabolic and Anti-Inflammatory Pathways
    Article Snippet: .. The primary antibodies used are rhodamine-conjugated WGA (Vector Laboratories, Newark, CA, USA, RL1022, 1:250), tdTomato (ORIGENE, Rockville, MD, USA, AB8181200, 1:200), Griffonia Simplicifolia Lectin I Isolectin B4 Fluorescein (Vector Laboratories, Newark, CA, USA, FL-1201, 1:50) and Alpha-SMA (Thermo Fisher Scientific, Waltham, MA, USA, PA5-85919, 1:200). .. After washing, the sections were incubated with the secondary antibodies at room temperature for 1 h. The secondary antibodies used are Rabbit anti-Goat IgG, Alexa Fluor TM 488 (Thermo Fisher Scientific, Waltham, MA, USA, A11078, 1:250) and Goat anti-Rabbit IgG, Alexa Fluor TM 594 (Thermo Fisher Scientific, Waltham, MA, USA, A11037, 1:250).

    Article Title: Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division
    Article Snippet: LipidSpotTM 488/610 Lipid Droplet Stains (#70065/#70069) and CF®488A, CF®640R or CF®555-conjugated wheat germ agglutinin (WGA; #29022, #29026 and #29076, respectively) as well as MitoViewTM Fix 640 (#70082), were obtained from Biotium Inc. (Fremont, CA). .. In some experiments Rhodamine-conjugated WGA (#RL-1022) from Vector Laboratories (Burlingame, CA) was used. .. Alexa Fluor®546 phalloidin (#A22283) was from Thermo Fisher Scientific.

    other:

    Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC-CXCL1-CXCR2 axis.
    Article Snippet: Sections were mounted with DAPI (1:1,000; D1306, Thermo Fisher Scientific) and incubated for 10 min at room temperature.

    Clinical Proteomics:

    Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC-CXCL1-CXCR2 axis.
    Article Snippet: For PDGFRα staining, a secondary antibody (goat anti-rabbit IgG [H + L] conjugated to Alexa Fluor 594 (1:200; A11012, Thermo Fisher Scientific)) was used, followed by incubation for 1 h at room temperature (20–25 °C). .. Rhodamine-conjugated WGA (1:200; RL-1022-5; Vector Laboratories) was used to visualize plasma membranes. .. Sections were mounted with DAPI (1:1,000; D1306, Thermo Fisher Scientific) and incubated for 10 min at room temperature.

    Article Title: Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC–CXCL1–CXCR2 axis
    Article Snippet: For PDGFRα staining, a secondary antibody (goat anti-rabbit IgG [H + L] conjugated to Alexa Fluor 594 (1:200; A11012, Thermo Fisher Scientific)) was used, followed by incubation for 1 h at room temperature (20–25 °C). .. Rhodamine-conjugated WGA (1:200; RL-1022-5; Vector Laboratories) was used to visualize plasma membranes. .. Sections were mounted with DAPI (1:1,000; D1306, Thermo Fisher Scientific) and incubated for 10 min at room temperature.

    Incubation:

    Article Title: Small molecule stimulators of steroid receptor coactivator-3 and methods of their use as cardioprotective and/or vascular regenerative agents
    Article Snippet: After that, antigen retrieval was performed (Antigen unmasking solution, Tris-based, Vector Labs cat #H-3301; Vector Labs, Burlingame, CA). .. Sections were permeabilized with 0.1% tween20-PBS, blocked with 10% donkey serum in 1% tween20-PBS, and then incubated with primary antibody in blocking solution (1:200 Rabbit anti-Lysozyme, abcam cat #AB108508; Abcam, Cambridge, United Kingdom), followed by secondary (1:200 Donkey anti-Rabbit, Alexa 647, Thermo Fisher Scientific cat #A-31573; Thermo Fisher Scientific, Waltham, MA), and then Rhodamine-conjugated WGA (1:250 Vector Labs Cat #RL-1022) and DAPI (1:500 Thermo Fisher Scientific Cat #62248). .. Images were taken on a Zeiss LSM780 confocal microscope.

    Blocking Assay:

    Article Title: Small molecule stimulators of steroid receptor coactivator-3 and methods of their use as cardioprotective and/or vascular regenerative agents
    Article Snippet: After that, antigen retrieval was performed (Antigen unmasking solution, Tris-based, Vector Labs cat #H-3301; Vector Labs, Burlingame, CA). .. Sections were permeabilized with 0.1% tween20-PBS, blocked with 10% donkey serum in 1% tween20-PBS, and then incubated with primary antibody in blocking solution (1:200 Rabbit anti-Lysozyme, abcam cat #AB108508; Abcam, Cambridge, United Kingdom), followed by secondary (1:200 Donkey anti-Rabbit, Alexa 647, Thermo Fisher Scientific cat #A-31573; Thermo Fisher Scientific, Waltham, MA), and then Rhodamine-conjugated WGA (1:250 Vector Labs Cat #RL-1022) and DAPI (1:500 Thermo Fisher Scientific Cat #62248). .. Images were taken on a Zeiss LSM780 confocal microscope.



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